Journal: Journal of Pain Research

Article Title: Expression of matrix metalloproteinases and components of the endocannabinoid system in the knee joint are associated with biphasic pain progression in a rat model of osteoarthritis

doi: 10.2147/JPR.S132682

Figure Lengend Snippet: Correlation matrix for DWB data and expression levels

Article Snippet: Cannabinoid receptor 2 , Cb2 , Rn04342831_s1 , 847 (NM_020543.4) , 99.

Techniques: Expressing, Gene Expression

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 activation reduces the number of infected cells without altering HIV receptor expression. a , b. Bulk RNA-seq analysis showing the expression of key endocannabinoid system genes in ( a ) MDMs and ( b ) iMg. c. Representative images of iMg immunostained for CD4 (red), CCR5 (green), and DAPI (blue). d. Quantification of CD4 and CCR5 expression. Each symbol represents the average of 2 wells/condition. n = 3 differentiations from 2 iMg lines. e. Representative images of iMg immunostained for HIV p24. f. Quantification of CD4 and CCR5 expression. Each symbol represents the average of 2 wells/condition. n = 3 differentiations from 2 iMg lines. One-way analysis of variance (ANOVA) with Dunnett’s correction for multiple comparisons. * p < 0.05; ns, not significant

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Activation Assay, Infection, Expressing, RNA Sequencing

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 agonists dose-dependently reduce HIV replication in macrophages and microglia. a , b. HIV p24 levels in supernatants of monocyte-derived macrophages (MDMs, n = 7) ( a ) and human induced pluripotent stem cell-derived microglia (iMg, n = 3) ( b ), normalized to the HIV + vehicle. Two-way ANOVA with Dunnett’s correction for multiple comparisons. * p < 0.05, ** p < 0.01. c , d. Bulk RNA-seq data showing count/million reads of key endocannabinoid system genes in MDMs ( c ) and iMg ( d )

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Derivative Assay, RNA Sequencing

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 agonists reduce global cytokine release following HIV infection in MDMs but not in iMg. a , b. Cytokine levels in cell-free supernatants of MDM ( a ) and ( b ) iMg cultures were measured with Bio-Plex Pro 48-Plex human cytokine screening panel. Heatmaps show log 2 fold changes from the HIV + vehicle condition. Supernatants were pooled from 4 wells/condition at days postinfection 9 (DPI 9) and assayed in duplicate. n = 6 MDM donors and 3 iMg lines. Student’s t test, ** p < 0.01. c. Levels of cytokines reportedly associated with HIV-induced brain injury in MDM and iMg supernatants, measured with Bio-Plex Pro 48-Plex human cytokine screening panel. d , e. Levels of tumor necrosis factor alpha (TNF-α) and IL-6 in ( d ) MDM and ( e ) iMg culture supernatants, measured with AlphaLISA. n = 6 MDM donors and 3 iMg lines. Student’s t test, * p < 0.05

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Infection

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: RNA-seq identifies convergent and divergent responses to CB 2 signaling between HIV-infected MDMs and iMg. a , b . Top 10 GO biological process gene sets enriched in the significant differentially expressed genes (DEGs) in comparison between HIV-infected/vehicle-treated and HIV-infected/JWH-133 treated ( a ) MDMs and ( b ) iMg. c. Top 10 KEGG gene sets enriched in the significant DEGs in comparison between HIV-infected/JWH-133-treated MDMs and iMg. n = 2 MDM donors and 2 iMg lines

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: RNA Sequencing, Infection, Comparison

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: CB 2 activation has minimal impact on transcriptional priming of the NLRP3 inflammasome in MDMs and iMg with established HIV infection. (a) NLRP3 inflammasome gene expression in MDMs on DPI 9, determined with qRT-PCR, represented as log 2 fold change from Mock + Veh. n = 3 MDM donors. (b) NLRP3 inflammasome gene expression in iMg on DPI 9, determined with bulk RNA-seq. n = 3 iMg lines

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Activation Assay, Infection, Gene Expression, Quantitative RT-PCR, RNA Sequencing

Journal: Journal of Neuroimmune Pharmacology

Article Title: Differential Effects of Cannabinoid Receptor 2 Agonists on HIV Replication and Inflammatory Activation in Monocyte-Derived Macrophages and Induced Pluripotent Stem Cell-Derived Microglia

doi: 10.1007/s11481-025-10254-x

Figure Lengend Snippet: In iMg, CB 2 -specific agonists impair HIV-induced NLRP3 inflammasome activation with a corresponding increase in autophagosome number. (a) Quantification of the number of NLRP3 + /ASC + specks in iMg detected with immunostaining. Average of 2 wells/condition from 3 differentiations in 2 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons. * p < 0.05. (b) Autophagic vacuoles measured by an autophagy assay. n = 3 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons, * p < 0.05. (c) Caspase 1 activity measured by the Caspase-Glo 1 inflammasome assay, with results normalized to HIV + Veh. n = 3 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons, * p < 0.05. (d) Levels of IL-1β in iMg supernatants, measured by AlphaLISA. n = 3 iMg lines, one-way ANOVA with Dunnett’s correction for multiple comparisons, ** p < 0.01; ns, not significant

Article Snippet: Custom TaqMan assay plates preloaded with the following human primer/probe pairs (Thermo Fisher Scientific, Waltham, MA) were used: GAPDH , Hs02786624_g1; CD4 , Hs01058407_m1, CCR5 , Hs99999149_s1; CXCR4 , Hs00607978_s1; CNR1 , Hs01038522_s1; CNR2 , Hs00361490_m1; GPR55 , Hs00271662_s1; IL1B , Hs01555410_m1; IL18 , Hs01038788_m1; NLRP3 , Hs00918082_m1; PYCARD , Hs01547324_gH; IL6 , Hs00174131_m1; TNFA , Hs00174128_m1; and CASP1 , Hs00354836_m1.

Techniques: Activation Assay, Immunostaining, Activity Assay

Journal: Clinical Cancer Research

Article Title: Cannabinoid Receptor Activation Induces Apoptosis through Tumor Necrosis Factor α–Mediated Ceramide De novo Synthesis in Colon Cancer Cells

doi: 10.1158/1078-0432.ccr-08-0799

Figure Lengend Snippet: Fig. 1. Expression of cannabinoid receptors in colon cancer cells and human specimens. A, Western blot analysis. Expression of CB1and CB2 receptor in the LoVo, DLD-1, and HT29 colon cancer cells, and in representative paired adenocarcinoma and adjacent normal mucosa from two patients.The samples were normalized for protein loading (70 Ag) by reblotting the membrane-bound protein with an antitubulin antibody.The densitometric histograms of relative band intensities are shown. Human spleen was used as a positive control for CB1and CB2 receptor expression. N, normal mucosa;T, tumor; kDa, kilodaltons. B, qualitative reverse transcription-PCR. Expression of CB1and CB2 receptor mRNA in the DLD-1and HT29 colon cancer cells (top), and in representative paired adenocarcinoma and adjacent normal mucosa from two patients (middle).The housekeeping gene GAPDH was used as an internal control (bottom). RNase-treated samples were used as negative controls. N, normal mucosa;T, tumor.

Article Snippet: The CB1 receptor agonist arachinodyl-2¶-chloroethylamide (ACEA), the CB1 antagonist AM251, and the CB2 antagonist AM630 were purchased from Tocris Bioscience.

Techniques: Expressing, Western Blot, Membrane, Positive Control, Reverse Transcription, Control

Journal: Clinical Cancer Research

Article Title: Cannabinoid Receptor Activation Induces Apoptosis through Tumor Necrosis Factor α–Mediated Ceramide De novo Synthesis in Colon Cancer Cells

doi: 10.1158/1078-0432.ccr-08-0799

Figure Lengend Snippet: Fig. 2. Expression of cannabinoid receptors in colon cancer cells and human specimens. A, immunofluorescence analysis of CB1and CB2 receptor expression in the DLD-1and HT29 colon cancer cells. Representative (63) images of cannabinoid receptor ^ stained colon cancer cells. Red, cannabinoid receptors; blue, nuclei. B, immunohistochemical analysis of CB1and CB2 receptors in two representative paired human cancer specimens and adjacent normal colon mucosa. Hematoxylin counterstain. Original magnification, 200.

Article Snippet: The CB1 receptor agonist arachinodyl-2¶-chloroethylamide (ACEA), the CB1 antagonist AM251, and the CB2 antagonist AM630 were purchased from Tocris Bioscience.

Techniques: Expressing, Immunofluorescence, Staining, Immunohistochemical staining

Journal: Clinical Cancer Research

Article Title: Cannabinoid Receptor Activation Induces Apoptosis through Tumor Necrosis Factor α–Mediated Ceramide De novo Synthesis in Colon Cancer Cells

doi: 10.1158/1078-0432.ccr-08-0799

Figure Lengend Snippet: Fig. 4. In vivo antitumor activity of CB13. A, Western blot analysis. Expression of CB2 receptor in tumors generated by s.c. injection of either DLD-1 (tumors1,11, and 12) or HT29 (tumors 4, 5, and 11) colon cancer cells in BALB/c mice.The samples were normalized for protein loading (70 Ag) by reblotting the membrane-bound protein with an antitubulin antibody.The densitometric histograms of relative band intensities are shown. Columns, mean of three different experiments; bars, SE. B, tumors generated by s.c. injection of DLD-1cells in BALB/c mice.The animals were treated with either vehicle or CB13 (2.5 mg/kg/d) for up to12 d (n = 8 for each experimental group).The tumor volume was monitored during the treatment. *, significantly different from vehicle-treated tumors at the corresponding day of treatment (P < 0.05). C, photographs of a representative vehicle-treated and CB13-treated tumor.

Article Snippet: The CB1 receptor agonist arachinodyl-2¶-chloroethylamide (ACEA), the CB1 antagonist AM251, and the CB2 antagonist AM630 were purchased from Tocris Bioscience.

Techniques: In Vivo, Activity Assay, Western Blot, Expressing, Generated, Injection, Membrane

Journal: Scientific Reports

Article Title: Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1

doi: 10.1038/s41598-023-32927-4

Figure Lengend Snippet: Cannabinoid receptor type 2 gene expression on HC69.5. HIV (GFP+) cells was assessed by RT PCR. H69.5 cells treated with/without 100 μg/ml Poly IC, 1 μM concentration of CBD or /and Δ (9)-THC. After 24 h of treatments, RNAs were extracted, and reverse transcribed followed by real time PCR for CNR2 gene (Hs00275635_m1; Thermofisher Scientific). GAPDH (Hs99999905_m1) was used as a housekeeping gene. Data represent the means ± standard error of three independent experiments. Graph represents the transcript accumulation index respect to negative control C20. Data were analyzed using GraphPad Prism software. The normality distribution was evaluated by Kolmogorov–Smirnov test, while statistical significance was calculated by ANOVA and Tukey Multiple Comparison Test as a post hoc test. Differences were considered significant at p ≤ 0.05.

Article Snippet: After 24 h of treatments, RNAs were extracted, and reverse transcribed followed by real time PCR for CNR2 gene (Hs00275635_m1; Thermofisher Scientific).

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control, Software, Comparison